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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant, insect-resistant eucalyptus
EN
955S019 × 1521K059
Yes
FGN-Ø3S19-5 × FGN-Ø6K59-4
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,Organization:FuturaGene Brasil Tecnologia Ltda ()Private sector (business and industry)
, BrazilPhone: + 55 15 3511 8032,Fax:Email: info@futuragene.com,Website: -
Organization:Suzano S.A. ()Escritório São Paulo Avenida Brigadeiro Faria Lima, 1355São Paulo, São Paulo
, BrazilPhone: +55 800 022 1727, +55 11 3503 9000,Fax:Email: suzanoresponde@suzano.com.br,Website: https://www.suzano.com.br/,
The eucalyptus (Eucalyptus urophylla) was modified for resistance to insects and tolerance to herbicides. For resistance to Lepidoptera, the modified eucalyptus expresses three Bacillus thuringiensis crystal proteins Cry1Ab, Cry1Bb and Cry2Aa, which have a pore forming mode-of-action in the epithelial lining of feeding larvae. For tolerance to glyphosate, the modified eucalyptus expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase, which does not bind the herbicide with high affinity and thus allows for the continued functioning of the shikimate pathway and biosynthesis of aromatic amino acids and compounds. In addition to these cassettes, the modified eucalyptus contains two Escherichia coli neomycin phosphotransferase II cassettes, which functioned as kanamycin selectable markers during transformation of the parental lines.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-272517-3 Organism Eucalyptus urophylla (Timor mountain gum, Timor white gum, Eucalyptus, EUCUR)Trees
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BCH-LMO-SCBD-272464-2 Living Modified Organism FGN-Ø3S19-5 - Herbicide-tolerant eucalyptusFuturaGene Brasil Tecnologia Ltda, Suzano S.A. | Resistance to antibiotics (Kanamycin, Neomycin), Resistance to herbicides (Glyphosate), Selectable marker genes and reporter genes
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BCH-LMO-SCBD-272537-1 Living Modified Organism FGN-Ø6K59-4 - Insect-resistant eucalyptusSuzano S.A., FuturaGene Brasil Tecnologia Ltda | Resistance to antibiotics (Kanamycin, Neomycin), Resistance to diseases and pests (Insects, Diptera (flies), Hessian fly (Mayetiola destructor), Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), European corn borer (Ostrinia nubilalis), Fall armyworm (Spodoptera frugiperda)), Selectable marker genes and reporter genes
EN
pBI121
EN
- Cross breeding
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-104820-3 Omega 5' untranslated leader | Tobacco mosaic virus (TMV)Leader
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-115762-1 Leader sequence | Tobacco etch virus (TEV)Leader sequence
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Promoter
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BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-272524-1 Ribulose-1,5-bisphosphate carboxylase/oxygenase small chain terminator | Eucalyptus grandis (Rose gum, Flooded gum, Grand eucalyptus, EUCGR)Terminator
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BCH-GENE-SCBD-272521-2 Cry1Bb | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), European corn borer (Ostrinia nubilalis), Fall armyworm (Spodoptera frugiperda))
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BCH-GENE-SCBD-272523-1 Ribulose-1,5-bisphosphate carboxylase/oxygenase small chain promoter | Eucalyptus grandis (Rose gum, Flooded gum, Grand eucalyptus, EUCGR)Promoter
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BCH-GENE-SCBD-272522-1 Cry2Aa | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Diptera (flies), Hessian fly (Mayetiola destructor), Lepidoptera (butterflies and moths), Cotton bollworm (Helicoverpa spp.), European corn borer (Ostrinia nubilalis), Fall armyworm (Spodoptera frugiperda))
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BCH-GENE-SCBD-272525-1 Elongation factor EF-1 alpha | Eucalyptus grandis (Rose gum, Flooded gum, Grand eucalyptus, EUCGR)Intron
DNA insert from 955S019 (FGN-Ø3S19-5)
The parental eucalyptus contains three gene cassettes: two Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (epsps) cassettes and one Escherichia coli neomycin phosphotransferase II (nptII) cassette.
The first epsps cassette is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an A. tumefaciens nopaline synthase terminator. This first cassette additionally contains a Tobacco mosaic virus omega leader sequence as a translational enhancer and an Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The second epsps cassette is under control of a Figwort mosaic virus 34S promoter and a nopaline synthase terminator. A chloroplast transit peptide 2 was also included to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The nptII sequence is under control of a CaMV 35S promoter and terminator. A Tobacco etch virus leader sequence was included to act as a translational enhancer. Due to the nature of the viral promoter, high levels of transcription are expected.
Note:
The first epsps cassette is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an A. tumefaciens nopaline synthase terminator. This first cassette additionally contains a Tobacco mosaic virus omega leader sequence as a translational enhancer and an Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The second epsps cassette is under control of a Figwort mosaic virus 34S promoter and a nopaline synthase terminator. A chloroplast transit peptide 2 was also included to direct the translated protein to the chloroplast. Due to the nature of the viral promoter, high levels of transcription are expected.
The nptII sequence is under control of a CaMV 35S promoter and terminator. A Tobacco etch virus leader sequence was included to act as a translational enhancer. Due to the nature of the viral promoter, high levels of transcription are expected.
Note:
- Molecular characterization indicated that a single T-DNA insertion occurred in the parental eucalyptus genome (in chromosome 3) without inclusion of vector backbone sequences. The sequences are present in the same arrangement as the transformation vector (i.e., without rearrangement). Protein expression was confirmed with ELISA.
DNA insert from 1521K059 (FGN-Ø6K59-4)
The parental eucalyptus contains four gene cassettes: Bacillus thuringiensis crystal protein Cry1Ab, B. thuringiensis crystal protein Cry1Bb, Escherichia coli neomycin phosphotransferase II (nptII) and B. thuringiensis crystal protein Cry2Aa.
Transcription of cry1Ab is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an Agrobacterium tumefaciens nopaline synthase (nos) terminator. A Tobacco mosaic virus omega 5' untranslated leader was also included to enhance translation of the Cry1Ab protein. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Transcription of cry1Bb is under control of a Eucalyptus grandis ribulose-1,5-bisphosphate carboxylase/oxygenase small chain promoter and terminator. The promoter contains the 5' untranslated region and intron to improve transcription. The promoter may be most active in green tissues.
Transcription of nptII is under control of a CaMV 35S promoter and terminator. A Tobacco etch virus leader sequence was also added to enhance translation of nptII. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Transcription of cry2Aa is under control of a CaMV 35S promoter and a nos terminator. An E. grandis elongation factor EF-1 alpha intron was included to enhance the expression of Cry2Aa. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Note:
Transcription of cry1Ab is under control of a Cauliflower mosaic virus (CaMV) 35S promoter and an Agrobacterium tumefaciens nopaline synthase (nos) terminator. A Tobacco mosaic virus omega 5' untranslated leader was also included to enhance translation of the Cry1Ab protein. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Transcription of cry1Bb is under control of a Eucalyptus grandis ribulose-1,5-bisphosphate carboxylase/oxygenase small chain promoter and terminator. The promoter contains the 5' untranslated region and intron to improve transcription. The promoter may be most active in green tissues.
Transcription of nptII is under control of a CaMV 35S promoter and terminator. A Tobacco etch virus leader sequence was also added to enhance translation of nptII. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Transcription of cry2Aa is under control of a CaMV 35S promoter and a nos terminator. An E. grandis elongation factor EF-1 alpha intron was included to enhance the expression of Cry2Aa. Due to the nature of the viral promoter, high levels of transcription are expected from this cassette.
Note:
- Next generation sequencing analyses indicated that parental eucalyptus genome contains a singular T-DNA insertion in chromosome 3 (located at approximately 66 Mbp) without integration of vector backbone sequences. The analyses also indicated that the four cassettes were present as expected (without rearrangements).
- ELISA analyses confirmed expression of Cry1Ab, Cry1Bb, Cry2Aa and NPTII in leaves (young and mature), stems, roots and pollen.
For more information, kindly refer to the parental LMO records.
EN
- Timber
- Other (Paper pulp)
EN
EN
